Isolation, point mutation of p5cs gene conferring drought tolerance and transformion into soybean varieties of vietnam

<p> 1.1. Soybean varieties collected are varied in phenotype, size and the number of seeds. Analysis of biochemical characteristics of local soybean varieties showed the strong correlation between the quality and the drougth tolerance. The correlation between the content of proline and the drought tolerance was proved in these studied soybean varieties. 1.2. The sequences of P5CS isolated in two soybean varieties DT84 and SL5 were 2148 nuleotide encoded for 715 amino acids. 1.3. The gene P5CS was mutated at the amino acid 125 in soybean varieties SL5. Asparated was replaced by alanine in protein sequence. 1.4. Promoter rd29A was succesfully isolated from Arabidopsis thaliana. Promoter rd29A was 1298bp in length and carried typical motifs including MYB, DRE, AMYBOX. </p>

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