Bước đầu hoàn thiện phương pháp và nghiên cứu sự đa dạng di truyền cây cóc trắng tại khu dự trữ sinh quyển rừng ngập mặn cần giờ bằng kỹ thuật rapd

This thesis was carried out in Can Gio Mangrove Biosphere Reserve Area and Chemical and Biological Analysis and Experiment Center Nong Lam University from April to August 2007. Lumnitzera racemosa Willd is one of 34 true mangrove species in Viet Nam. In Can Gio Mangrove Biosphere Reserve Area, it is a common population. Almost the population is naturally regenerated after the war. However, after 30 years, the population has degraduated. In order to protect and preserve the Can Gio mangrove forest, scheme for afforestation and conservation should be established. Studies on population genetics are necessary to provide basic information for establishing strategies for conserving genetic diversity of Can Gio mangrove forest. The obtained results are:  Collected 45 leaf samples from Lumnitzera racemosa.  Optimized DNA isolation protocol.  Developed RAPD protocol using for Lumnitzera racemosa. Primer OPAC10 showed the genetic diversity clearlier than other tested primers. We had 4 polymorphic fragments and 2 isomorphic fragments. It is possible to be DNA marker for Lumnitzera racemosa species.  The phylogenic tree of Lumnitzera racemosa divided 11 samples into 2 group. The first group had 2 samples which have been collected along road, the others have been collected along river.  The Lumnitzera racemosa population in Can Gio Mangrove Biosphere Reserve had low level of genetic diversity. In order to increase the level of genetic diversity, we should plant Lumnitzera racemosa with different origin into the existing population.



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